The metabolism of ethanol in rat-liver suspensions.

3. Bile and urine samples were hydrolysed by standard methods: metabolites excreted included unconjugated material, glucuronides and substances hydrolysed by cold and hot acid; the major part of the excreted radioactivity was present as substances remaining water-soluble after all forms of hydrolysis. 4. When samples of bile were collected over periods of 1 hr. or less the proportion of radioactivity excreted as glucuronide always decreased in successive samples whereas water-soluble metabolites generally increased. Substances hydrolysed by cold acid remained virtually constant whereas those hydrolysed by hot acid showed no consistent change. 5. The relationship of these results to similar studies of progesterone metabolism in the cat and of other steroids in man and animals is discussed.